Gram stain
Gram-positive bacteria
Bacteria that keep the crystal-violet stain and read purple under the microscope.
Why they stain this way
Gram-positive cell walls carry a thick peptidoglycan layer that traps the crystal violet-iodine complex through the alcohol decolourisation step, so the cells stay purple and never take up the safranin counterstain.
That same thick wall is why the classic Gram-positive drugs work the way they do, and why arrangement - clusters versus chains, cocci versus rods - is usually the next branch after the stain itself.
PetriKey lists 23 Gram-positive bacteria. Only bacteria appear here - lab methods and antimicrobials carry gram associations rather than a stain of their own, so they are filed under lab & diagnostics and antimicrobials.
Browse by shape
Shape is the next branch after the stain. Each Gram-positive shape group has its own study page with the identifying lab clues for every member.
All 23
Gram-positive organisms
Actinomyces israelii
Sulfur-granule filamentous anaerobe
Gram +Bacillus anthracis
Spore-forming aerobic Gram-positive rod
Gram +Bacillus cereus
Reheated-rice food poisoning rod
Gram +Clostridioides difficile
Spore-forming anaerobic rod
Gram +Clostridium botulinum
Flaccid-paralysis canned-food anaerobe
Gram +Clostridium perfringens
Anaerobic spore-forming rod
Gram +Clostridium tetani
Terminal-spore tetanus rod
Gram +Corynebacterium diphtheriae
Club-shaped Gram-positive rod with toxin clue
Gram +Cutibacterium acnes
Acne & prosthesis anaerobe (formerly Propionibacterium)
Gram +Enterococcus faecalis/faecium
Bile-esculin and VRE vocabulary
Gram +Lactobacillus species
Doderlein vaginal-flora acid producer
Gram +Listeria monocytogenes
Tumbling motility Gram-positive rod
Gram +Staphylococcus aureus
Gram-positive cocci in clusters
Gram +Staphylococcus epidermidis
Coagulase-negative skin flora clue
Gram +Staphylococcus saprophyticus
Coagulase-negative, novobiocin-resistant clue
Gram +Streptococcus agalactiae
Group B beta-hemolytic streptococcus
Gram +Streptococcus anginosus group
Abscess-forming viridans (milleri) streptococci
Gram +Streptococcus gallolyticus (bovis)
Group D strep linked to colon cancer
Gram +Streptococcus mutans
Dental caries viridans streptococcus
Gram +Streptococcus pneumoniae
Lancet diplococci and capsule clue
Gram +Streptococcus pyogenes
Group A beta-hemolytic streptococcus
Gram +Tropheryma whipplei
Actinobacterium of PAS-positive Whipple disease
Gram +Viridans streptococci
Alpha-hemolytic optochin-resistant oral strep
Gram +