Skip to content
PetriKey

Gram stain

Gram-positive bacteria

Bacteria that keep the crystal-violet stain and read purple under the microscope.

Why they stain this way

Gram-positive cell walls carry a thick peptidoglycan layer that traps the crystal violet-iodine complex through the alcohol decolourisation step, so the cells stay purple and never take up the safranin counterstain.

That same thick wall is why the classic Gram-positive drugs work the way they do, and why arrangement - clusters versus chains, cocci versus rods - is usually the next branch after the stain itself.

PetriKey lists 23 Gram-positive bacteria. Only bacteria appear here - lab methods and antimicrobials carry gram associations rather than a stain of their own, so they are filed under lab & diagnostics and antimicrobials.

Browse by shape

Shape is the next branch after the stain. Each Gram-positive shape group has its own study page with the identifying lab clues for every member.

All 23

Gram-positive organisms

Full library →
Bacterium

Actinomyces israelii

Sulfur-granule filamentous anaerobe

Gram +
Bacterium

Bacillus anthracis

Spore-forming aerobic Gram-positive rod

Gram +
Bacterium

Bacillus cereus

Reheated-rice food poisoning rod

Gram +
Bacterium

Clostridioides difficile

Spore-forming anaerobic rod

Gram +
Bacterium

Clostridium botulinum

Flaccid-paralysis canned-food anaerobe

Gram +
Bacterium

Clostridium perfringens

Anaerobic spore-forming rod

Gram +
Bacterium

Clostridium tetani

Terminal-spore tetanus rod

Gram +
Bacterium

Corynebacterium diphtheriae

Club-shaped Gram-positive rod with toxin clue

Gram +
Bacterium

Cutibacterium acnes

Acne & prosthesis anaerobe (formerly Propionibacterium)

Gram +
Bacterium

Enterococcus faecalis/faecium

Bile-esculin and VRE vocabulary

Gram +
Bacterium

Lactobacillus species

Doderlein vaginal-flora acid producer

Gram +
Bacterium

Listeria monocytogenes

Tumbling motility Gram-positive rod

Gram +
Bacterium

Staphylococcus aureus

Gram-positive cocci in clusters

Gram +
Bacterium

Staphylococcus epidermidis

Coagulase-negative skin flora clue

Gram +
Bacterium

Staphylococcus saprophyticus

Coagulase-negative, novobiocin-resistant clue

Gram +
Bacterium

Streptococcus agalactiae

Group B beta-hemolytic streptococcus

Gram +
Bacterium

Streptococcus anginosus group

Abscess-forming viridans (milleri) streptococci

Gram +
Bacterium

Streptococcus gallolyticus (bovis)

Group D strep linked to colon cancer

Gram +
Bacterium

Streptococcus mutans

Dental caries viridans streptococcus

Gram +
Bacterium

Streptococcus pneumoniae

Lancet diplococci and capsule clue

Gram +
Bacterium

Streptococcus pyogenes

Group A beta-hemolytic streptococcus

Gram +
Bacterium

Tropheryma whipplei

Actinobacterium of PAS-positive Whipple disease

Gram +
Bacterium

Viridans streptococci

Alpha-hemolytic optochin-resistant oral strep

Gram +